SOP – Preparation and maintenance of tumour-infiltrating lymphocyte, cancer associated fibroblast and autologous tumour cultures (ovarian cancer)

The purpose of this document is to provide clear guidance on the procedures to be followed during the processing of ovarian tumour tissue to establish a) a tumour infiltrating lymphocyte culture; (b) an autologous tumour cell culture; (c) a cryopreserved aliquot of tumour culture digest; (d) subsequent cryopreservation of TIL and autologous tumour cultures for future assays.